Positive Control:Article Title: Generation and characterization of iPSC-derived renal proximal tubule-like cells with extended stability
Article Snippet: .. Cells were used as positive control for uromodulin expression. (Jennings et al. 2007) HepG2 Human liver cancer cell line Commonly used cell in molecular biology and toxicology experiments. (van der Stel et al. 2020) Supplementary Table S2 Antibody/dye Company Catalogue number Concentration/ Dilution Application ACE2 R&D systems AF933-SP 1:250 IF (immunofluorescence) Alpha acetylated tubulin Invitrogen 32-2700 1:200 IF Anti - Goat IgG - Alexa 546 Invitrogen A-11056 1:1000 IF Anti - Mouse IgG - Alexa 488 Invitrogen A-21202 1:1000 IF Anti - Mouse IgG - HRP Invitrogen 31430 1:10000 WB (western blot) Anti - Rabbit IgG - Alexa 546 Invitrogen A10040 1:1000 IF Anti - Rabbit IgG - AlexaFluor 488 Invitrogen A-21206 1:1000 IF Anti - Rabbit IgG - HRP Invitrogen 31460 1:10000 WB BSA – Alexa Fluor 647 Invitrogen A34785 10 μg/ml Albumin uptake assay (Albumin as a substrate for Megalin) Calcein-AM Sigma-Aldrich 239835 5 μM Calcein-AM efflux assay (Calcein-AM as a substrate for p-GP) DLL1 Abcam ab84620 1:1000 WB HNF4A Sigma HPA004712 1:50 IF Hoechst 33342 Invitrogen H3570 1 μg/ml IF (nuclear staining) Megalin R&D systems MAB9578 1:100 IF Megalin Abcam ab76969 1:1000 WB Nephrin Abcam ab183099 1:100 IF Occludin – Alexa Fluor 594 Invitrogen 331594 1:250 IF Oct 3/4 Santacruz SC-5279 1:100/1:1000 IF/WB PAX2 Abcam ab79389 1:100 IF Phalloidin-Alexa Fluor 488 Invitrogen R37110 1 drop/ml IF (F-actin staining) Tubulin Abcam ab4074 1:1000 WB UMOD Cedarlane CL1032A 1:500 IF WT1 R&D systems AF5729 1:100 IF ZO3 Invitrogen 36-4000 1:250 IF Supplementary Figure legends Figure S1: Immunofluorescence imaging showing the phenotype of proximal tubular-like cells SBAD3 and SFC-086 were differentiated into PTL on Geltrex and cells were fixed on day 0, day 6 and day 16 of differentiation and stained with antibodies for selected markers. ..
Expressing:Article Title: Generation and characterization of iPSC-derived renal proximal tubule-like cells with extended stability
Article Snippet: .. Cells were used as positive control for uromodulin expression. (Jennings et al. 2007) HepG2 Human liver cancer cell line Commonly used cell in molecular biology and toxicology experiments. (van der Stel et al. 2020) Supplementary Table S2 Antibody/dye Company Catalogue number Concentration/ Dilution Application ACE2 R&D systems AF933-SP 1:250 IF (immunofluorescence) Alpha acetylated tubulin Invitrogen 32-2700 1:200 IF Anti - Goat IgG - Alexa 546 Invitrogen A-11056 1:1000 IF Anti - Mouse IgG - Alexa 488 Invitrogen A-21202 1:1000 IF Anti - Mouse IgG - HRP Invitrogen 31430 1:10000 WB (western blot) Anti - Rabbit IgG - Alexa 546 Invitrogen A10040 1:1000 IF Anti - Rabbit IgG - AlexaFluor 488 Invitrogen A-21206 1:1000 IF Anti - Rabbit IgG - HRP Invitrogen 31460 1:10000 WB BSA – Alexa Fluor 647 Invitrogen A34785 10 μg/ml Albumin uptake assay (Albumin as a substrate for Megalin) Calcein-AM Sigma-Aldrich 239835 5 μM Calcein-AM efflux assay (Calcein-AM as a substrate for p-GP) DLL1 Abcam ab84620 1:1000 WB HNF4A Sigma HPA004712 1:50 IF Hoechst 33342 Invitrogen H3570 1 μg/ml IF (nuclear staining) Megalin R&D systems MAB9578 1:100 IF Megalin Abcam ab76969 1:1000 WB Nephrin Abcam ab183099 1:100 IF Occludin – Alexa Fluor 594 Invitrogen 331594 1:250 IF Oct 3/4 Santacruz SC-5279 1:100/1:1000 IF/WB PAX2 Abcam ab79389 1:100 IF Phalloidin-Alexa Fluor 488 Invitrogen R37110 1 drop/ml IF (F-actin staining) Tubulin Abcam ab4074 1:1000 WB UMOD Cedarlane CL1032A 1:500 IF WT1 R&D systems AF5729 1:100 IF ZO3 Invitrogen 36-4000 1:250 IF Supplementary Figure legends Figure S1: Immunofluorescence imaging showing the phenotype of proximal tubular-like cells SBAD3 and SFC-086 were differentiated into PTL on Geltrex and cells were fixed on day 0, day 6 and day 16 of differentiation and stained with antibodies for selected markers. ..
Concentration Assay:Article Title: Generation and characterization of iPSC-derived renal proximal tubule-like cells with extended stability
Article Snippet: .. Cells were used as positive control for uromodulin expression. (Jennings et al. 2007) HepG2 Human liver cancer cell line Commonly used cell in molecular biology and toxicology experiments. (van der Stel et al. 2020) Supplementary Table S2 Antibody/dye Company Catalogue number Concentration/ Dilution Application ACE2 R&D systems AF933-SP 1:250 IF (immunofluorescence) Alpha acetylated tubulin Invitrogen 32-2700 1:200 IF Anti - Goat IgG - Alexa 546 Invitrogen A-11056 1:1000 IF Anti - Mouse IgG - Alexa 488 Invitrogen A-21202 1:1000 IF Anti - Mouse IgG - HRP Invitrogen 31430 1:10000 WB (western blot) Anti - Rabbit IgG - Alexa 546 Invitrogen A10040 1:1000 IF Anti - Rabbit IgG - AlexaFluor 488 Invitrogen A-21206 1:1000 IF Anti - Rabbit IgG - HRP Invitrogen 31460 1:10000 WB BSA – Alexa Fluor 647 Invitrogen A34785 10 μg/ml Albumin uptake assay (Albumin as a substrate for Megalin) Calcein-AM Sigma-Aldrich 239835 5 μM Calcein-AM efflux assay (Calcein-AM as a substrate for p-GP) DLL1 Abcam ab84620 1:1000 WB HNF4A Sigma HPA004712 1:50 IF Hoechst 33342 Invitrogen H3570 1 μg/ml IF (nuclear staining) Megalin R&D systems MAB9578 1:100 IF Megalin Abcam ab76969 1:1000 WB Nephrin Abcam ab183099 1:100 IF Occludin – Alexa Fluor 594 Invitrogen 331594 1:250 IF Oct 3/4 Santacruz SC-5279 1:100/1:1000 IF/WB PAX2 Abcam ab79389 1:100 IF Phalloidin-Alexa Fluor 488 Invitrogen R37110 1 drop/ml IF (F-actin staining) Tubulin Abcam ab4074 1:1000 WB UMOD Cedarlane CL1032A 1:500 IF WT1 R&D systems AF5729 1:100 IF ZO3 Invitrogen 36-4000 1:250 IF Supplementary Figure legends Figure S1: Immunofluorescence imaging showing the phenotype of proximal tubular-like cells SBAD3 and SFC-086 were differentiated into PTL on Geltrex and cells were fixed on day 0, day 6 and day 16 of differentiation and stained with antibodies for selected markers. ..
Immunofluorescence:Article Title: Generation and characterization of iPSC-derived renal proximal tubule-like cells with extended stability
Article Snippet: .. Cells were used as positive control for uromodulin expression. (Jennings et al. 2007) HepG2 Human liver cancer cell line Commonly used cell in molecular biology and toxicology experiments. (van der Stel et al. 2020) Supplementary Table S2 Antibody/dye Company Catalogue number Concentration/ Dilution Application ACE2 R&D systems AF933-SP 1:250 IF (immunofluorescence) Alpha acetylated tubulin Invitrogen 32-2700 1:200 IF Anti - Goat IgG - Alexa 546 Invitrogen A-11056 1:1000 IF Anti - Mouse IgG - Alexa 488 Invitrogen A-21202 1:1000 IF Anti - Mouse IgG - HRP Invitrogen 31430 1:10000 WB (western blot) Anti - Rabbit IgG - Alexa 546 Invitrogen A10040 1:1000 IF Anti - Rabbit IgG - AlexaFluor 488 Invitrogen A-21206 1:1000 IF Anti - Rabbit IgG - HRP Invitrogen 31460 1:10000 WB BSA – Alexa Fluor 647 Invitrogen A34785 10 μg/ml Albumin uptake assay (Albumin as a substrate for Megalin) Calcein-AM Sigma-Aldrich 239835 5 μM Calcein-AM efflux assay (Calcein-AM as a substrate for p-GP) DLL1 Abcam ab84620 1:1000 WB HNF4A Sigma HPA004712 1:50 IF Hoechst 33342 Invitrogen H3570 1 μg/ml IF (nuclear staining) Megalin R&D systems MAB9578 1:100 IF Megalin Abcam ab76969 1:1000 WB Nephrin Abcam ab183099 1:100 IF Occludin – Alexa Fluor 594 Invitrogen 331594 1:250 IF Oct 3/4 Santacruz SC-5279 1:100/1:1000 IF/WB PAX2 Abcam ab79389 1:100 IF Phalloidin-Alexa Fluor 488 Invitrogen R37110 1 drop/ml IF (F-actin staining) Tubulin Abcam ab4074 1:1000 WB UMOD Cedarlane CL1032A 1:500 IF WT1 R&D systems AF5729 1:100 IF ZO3 Invitrogen 36-4000 1:250 IF Supplementary Figure legends Figure S1: Immunofluorescence imaging showing the phenotype of proximal tubular-like cells SBAD3 and SFC-086 were differentiated into PTL on Geltrex and cells were fixed on day 0, day 6 and day 16 of differentiation and stained with antibodies for selected markers. ..
Western Blot:Article Title: Generation and characterization of iPSC-derived renal proximal tubule-like cells with extended stability
Article Snippet: .. Cells were used as positive control for uromodulin expression. (Jennings et al. 2007) HepG2 Human liver cancer cell line Commonly used cell in molecular biology and toxicology experiments. (van der Stel et al. 2020) Supplementary Table S2 Antibody/dye Company Catalogue number Concentration/ Dilution Application ACE2 R&D systems AF933-SP 1:250 IF (immunofluorescence) Alpha acetylated tubulin Invitrogen 32-2700 1:200 IF Anti - Goat IgG - Alexa 546 Invitrogen A-11056 1:1000 IF Anti - Mouse IgG - Alexa 488 Invitrogen A-21202 1:1000 IF Anti - Mouse IgG - HRP Invitrogen 31430 1:10000 WB (western blot) Anti - Rabbit IgG - Alexa 546 Invitrogen A10040 1:1000 IF Anti - Rabbit IgG - AlexaFluor 488 Invitrogen A-21206 1:1000 IF Anti - Rabbit IgG - HRP Invitrogen 31460 1:10000 WB BSA – Alexa Fluor 647 Invitrogen A34785 10 μg/ml Albumin uptake assay (Albumin as a substrate for Megalin) Calcein-AM Sigma-Aldrich 239835 5 μM Calcein-AM efflux assay (Calcein-AM as a substrate for p-GP) DLL1 Abcam ab84620 1:1000 WB HNF4A Sigma HPA004712 1:50 IF Hoechst 33342 Invitrogen H3570 1 μg/ml IF (nuclear staining) Megalin R&D systems MAB9578 1:100 IF Megalin Abcam ab76969 1:1000 WB Nephrin Abcam ab183099 1:100 IF Occludin – Alexa Fluor 594 Invitrogen 331594 1:250 IF Oct 3/4 Santacruz SC-5279 1:100/1:1000 IF/WB PAX2 Abcam ab79389 1:100 IF Phalloidin-Alexa Fluor 488 Invitrogen R37110 1 drop/ml IF (F-actin staining) Tubulin Abcam ab4074 1:1000 WB UMOD Cedarlane CL1032A 1:500 IF WT1 R&D systems AF5729 1:100 IF ZO3 Invitrogen 36-4000 1:250 IF Supplementary Figure legends Figure S1: Immunofluorescence imaging showing the phenotype of proximal tubular-like cells SBAD3 and SFC-086 were differentiated into PTL on Geltrex and cells were fixed on day 0, day 6 and day 16 of differentiation and stained with antibodies for selected markers. ..
Staining:Article Title: Generation and characterization of iPSC-derived renal proximal tubule-like cells with extended stability
Article Snippet: .. Cells were used as positive control for uromodulin expression. (Jennings et al. 2007) HepG2 Human liver cancer cell line Commonly used cell in molecular biology and toxicology experiments. (van der Stel et al. 2020) Supplementary Table S2 Antibody/dye Company Catalogue number Concentration/ Dilution Application ACE2 R&D systems AF933-SP 1:250 IF (immunofluorescence) Alpha acetylated tubulin Invitrogen 32-2700 1:200 IF Anti - Goat IgG - Alexa 546 Invitrogen A-11056 1:1000 IF Anti - Mouse IgG - Alexa 488 Invitrogen A-21202 1:1000 IF Anti - Mouse IgG - HRP Invitrogen 31430 1:10000 WB (western blot) Anti - Rabbit IgG - Alexa 546 Invitrogen A10040 1:1000 IF Anti - Rabbit IgG - AlexaFluor 488 Invitrogen A-21206 1:1000 IF Anti - Rabbit IgG - HRP Invitrogen 31460 1:10000 WB BSA – Alexa Fluor 647 Invitrogen A34785 10 μg/ml Albumin uptake assay (Albumin as a substrate for Megalin) Calcein-AM Sigma-Aldrich 239835 5 μM Calcein-AM efflux assay (Calcein-AM as a substrate for p-GP) DLL1 Abcam ab84620 1:1000 WB HNF4A Sigma HPA004712 1:50 IF Hoechst 33342 Invitrogen H3570 1 μg/ml IF (nuclear staining) Megalin R&D systems MAB9578 1:100 IF Megalin Abcam ab76969 1:1000 WB Nephrin Abcam ab183099 1:100 IF Occludin – Alexa Fluor 594 Invitrogen 331594 1:250 IF Oct 3/4 Santacruz SC-5279 1:100/1:1000 IF/WB PAX2 Abcam ab79389 1:100 IF Phalloidin-Alexa Fluor 488 Invitrogen R37110 1 drop/ml IF (F-actin staining) Tubulin Abcam ab4074 1:1000 WB UMOD Cedarlane CL1032A 1:500 IF WT1 R&D systems AF5729 1:100 IF ZO3 Invitrogen 36-4000 1:250 IF Supplementary Figure legends Figure S1: Immunofluorescence imaging showing the phenotype of proximal tubular-like cells SBAD3 and SFC-086 were differentiated into PTL on Geltrex and cells were fixed on day 0, day 6 and day 16 of differentiation and stained with antibodies for selected markers. ..
Imaging:Article Title: Generation and characterization of iPSC-derived renal proximal tubule-like cells with extended stability
Article Snippet: .. Cells were used as positive control for uromodulin expression. (Jennings et al. 2007) HepG2 Human liver cancer cell line Commonly used cell in molecular biology and toxicology experiments. (van der Stel et al. 2020) Supplementary Table S2 Antibody/dye Company Catalogue number Concentration/ Dilution Application ACE2 R&D systems AF933-SP 1:250 IF (immunofluorescence) Alpha acetylated tubulin Invitrogen 32-2700 1:200 IF Anti - Goat IgG - Alexa 546 Invitrogen A-11056 1:1000 IF Anti - Mouse IgG - Alexa 488 Invitrogen A-21202 1:1000 IF Anti - Mouse IgG - HRP Invitrogen 31430 1:10000 WB (western blot) Anti - Rabbit IgG - Alexa 546 Invitrogen A10040 1:1000 IF Anti - Rabbit IgG - AlexaFluor 488 Invitrogen A-21206 1:1000 IF Anti - Rabbit IgG - HRP Invitrogen 31460 1:10000 WB BSA – Alexa Fluor 647 Invitrogen A34785 10 μg/ml Albumin uptake assay (Albumin as a substrate for Megalin) Calcein-AM Sigma-Aldrich 239835 5 μM Calcein-AM efflux assay (Calcein-AM as a substrate for p-GP) DLL1 Abcam ab84620 1:1000 WB HNF4A Sigma HPA004712 1:50 IF Hoechst 33342 Invitrogen H3570 1 μg/ml IF (nuclear staining) Megalin R&D systems MAB9578 1:100 IF Megalin Abcam ab76969 1:1000 WB Nephrin Abcam ab183099 1:100 IF Occludin – Alexa Fluor 594 Invitrogen 331594 1:250 IF Oct 3/4 Santacruz SC-5279 1:100/1:1000 IF/WB PAX2 Abcam ab79389 1:100 IF Phalloidin-Alexa Fluor 488 Invitrogen R37110 1 drop/ml IF (F-actin staining) Tubulin Abcam ab4074 1:1000 WB UMOD Cedarlane CL1032A 1:500 IF WT1 R&D systems AF5729 1:100 IF ZO3 Invitrogen 36-4000 1:250 IF Supplementary Figure legends Figure S1: Immunofluorescence imaging showing the phenotype of proximal tubular-like cells SBAD3 and SFC-086 were differentiated into PTL on Geltrex and cells were fixed on day 0, day 6 and day 16 of differentiation and stained with antibodies for selected markers. ..
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